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  • br Conclusions br In the present


    5. Conclusions
    In the present study, a new type of PAMAM dendrimer deriva-tive was established. HA was used as the targeting group, and PAMAM was coated with Pt and Dox by a covalent reaction, which were found highly stable, biocompatible, and potent against breast cancer. This design enabled the synergistic killing of breast cancer cells. The dual drug-loaded [email protected] was found to be very stable and entered AZD-5991 through the lysosome-mediate path-way. Cytotoxicity studies were employed in MCF-7 and MDA-MB-231 breast cancer cells, which indicated that [email protected] had a higher anticancer effect than that of monomers. In vivo tissue distribution studies of [email protected] demonstrated that [email protected] showed a remarkable increase in drug accumulation in the tumor tissue when compared with free drug solutions. Moreover, drug distribution studies indicated that after 24 h, almost no drugs were observed in the heart and kidneys, thus indicating that [email protected] may decrease side effects and increase antitumor effi-ciency. The in vivo antitumor efficacy data using MDA-MB-231 tumor-bearing BALB/c mice demonstrated that [email protected] had a stronger antitumor effect than other drug formulations. The present study demonstrated the co-delivery of Pt with Dox to achieve highly efficient ‘‘synergism” in breast cancer cells that may be extended to the other anticancer drugs.
    Declaration of interest
    The authors declare that there is no conflict of interest regard-ing the publication of this paper.
    Appendix A. Supplementary data
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